Medium-pressure Liquid Chromatography

DMEM Dulbecco’s Modified Eagle’s Medium DMSO dimethylsulfoxide HLM human liver microsomes HPLC high-performance liquid chromatography IVIVE in vitro-in vivo extrapolation

Ascorbic Acid Quantification in Melon Samples – the Importance of the Extraction Medium for Hplc Analysis

Two high-performance liquid chromatography (HPLC) methods for ascorbic acid (AA) quantification were compared in three cultivars of melon (Cucumis melo L.) fruits. Both procedures were analogous except for the extraction medium used: 6 % meta-phosphoric acid and 3 % citric acid. The general conclusion is that 3 % citric acid is a better extraction medium compared to 6 % meta-phosphoric acid and...

Increase in biodegradation of paclobutrazol in soils

Background Paclobutrazol is a regulator of vegetal growth that remains active in the soil and can affect the growth and the development of subsequent cultures. Experiments in two soils (A and B) with and without addition of inoculum or mineral medium were performed to investigate the biodegradation of paclobutrazol. The inoculum contained a mixed culture of three strains of bacteria isolated by...

ON-LINE SOLID-PHASE EXTRACTION AND LIQUID CHROMATOGRAPHY/PARTICLE BEAM-MASS SPECTROMETRY FOR DEGRADATION STUDIES OF SOME POLAR PESTICIDES IN WATER

An on-line automated method for photodegradation studies of isoproturon, diuron, atrazine, fenitrothion, and metoxuron by means of liquid chromatography/mass spectrometry (LC/MS) with particle beam (PB) interface is described. Surface water samples were first spiked with 50 µg/l of each pesticide and then exposed to the radiation of the medium-pressure mercury lamp. Next, in regular intervals o...

Inhibitors of sterol synthesis. Chromatography of acetate derivatives of oxygenated sterols.

The separation of the acetate derivatives of a number of oxygenated sterols was achieved by medium pressure liquid chromatography on silica gel columns and by normal and reversed phase high performance liquid chromatography. We have explored the application of these chromatographic systems for the analysis of oxygenated sterols of plasma samples from two normal human subjects. The addition of h...